Table 2. Comparison of divergence to the reference sequence calculated using sanger PCR sequences, RAR, and the assemblies produced by Pool et al. [25].

Line	Assembly	Bases Aligned	SNPs	π
ZK84	PCR	52099	568	0.0109
	RAR	51456	557	0.0108
	BWA DPGP2	48553	398	0.0082
ZK131	PCR	52134	561	0.0108
	RAR	51540	553	0.0107
	BWA DPGP2	49060	422	0.0086
ZK186	PCR	51985	642	0.0123
	RAR	51300	636	0.0124
	BWA DPGP2	48454	433	0.0089