Table 1. Overview of the mutations present in the recH342 strain identified by nucleotide sequencing.

Genome positiona	Locusa	Gene	Gene product	Referenceb	Testb	Amino acid changec
330380	329774–330739	ldh	L-lactate dehydrogenase	AAA (607)	GAA	K203E
925934	925633–926427	yfhG	Hypothetical recombination regulator, RecX	CTT (302)	CCT	L101P
1041854	1041994–1042842	dat	Intergenic, D-alanine aminotransferase	T	G	-
1283580	1283463–1284362	yjfC	Hypothetical protein BSU12130	ACC	GCC	T40A
2893906	c2894709–2893681	ilvC	Ketol-acid reductoisomerase	GCG (207)	GCA	A69A (silent)
2982417	c2982269–2981151	citZ	Intergenic, citrate synthase	T	A	-
2982437	c2983067–2982603	ytwI	Intergenic, hypothetical protein	T	C	-
3024588	c3025266–3023677	ytcI	Acyl-CoA synthetase	GTA (685)	ATA	V227I
3051461	c3052583–3049725	sftA	DNA translocase	CCA (1123)	TCA	P236S

^aThe single genome position presenting the mutated nucleotide position, and the locus interval are shown, where the c denotes the complementary strand.

^bThe underlined bases represent the bases that were present in the Reference (BG214) and the Test (recH342) sample, and between parentheses the exchanged nucleotide number.

^cThe position and substitution of the mutated residue.