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. 2012 Dec 20;8(12):e1003126. doi: 10.1371/journal.pgen.1003126

Table 2. RecX alone, or in concert with RecO or RecF, plays an important role in chromosomal and plasmid transformation.

Strain % Chromosomal transformationa % Plasmid Transformationb
rec + 100 (5.7×10−3) 100 (4.7×10−5)
recF15 c 71.4 96.2
ΔrecR c 69.0 94.1
ΔrecO c 58.2 3.2
recX342c 60.9 93
recX342 ΔrecO c <0.1 0.1
recX342 ΔrecR c <0.1 0.9
recX342 recF15 c <0.1 <0.1
ΔrecA c <0.01 97.4
ΔrecX 0.5 1.8
ΔrecX ΔrecA <0.01 57
ΔrecX ΔrecO <0.1 <0.1
ΔrecX recF15 <0.1 <0.1

The metB5 locus contains a single point mutation.

a

The yield of met + transformants (SB19 DNA, chromosomal transformation) and

b

kanamycin resistant transformants (pUB110, plasmid transformation) was corrected for DNA uptake and cell viability, and the values obtained normalized relative to that of the rec + strain, taken as 100. Between parentheses is included the number of transformants/cell.

c

The transformation frequencies of rec +, recF15, ΔrecR, ΔrecO, recX342 (previously termed recH342), recX342 ΔrecR, recX342 recF15, recX342 ΔrecO, recX342 ΔrecR and ΔrecA cells were reported elsewhere [13], [15], [16], [52] and determined here for direct comparison. The results are the average of at least three independent experiments and are within a 10% standard error.