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. 2012 Dec 20;8(12):e1003130. doi: 10.1371/journal.pgen.1003130

Table 2. List of primers used for expression studies.

Primers used for cDNA synthesis Primer sequence (5′ - 3′) used for PCR or qRT
Experiment: Sequencing PEP1 cDNAs from accessions ( Figure 3E , Table 1 , Table S2)
Oligo-dT PEP1_5UTRF1: AACGCTTAGTATCTCAGGCGACPEP1_5UTRF2: CCTTCTCGGAGACAGAAGCCPEP1_3UTRR1: AGTCTCTCAGCCATAGAGAG
Experiment: PEP1 expression ( Figure 2A , PEP1 primers as in [2] )
Oligo-dT PEP1(a+b)F: CTTGTCGTCTCCTCCTCTGGPEP1(a+b)R: ACTACGGCGAGAGCAGTTTCAaPP2AF: AGTATCGCTTCTCGCTCCAGAaPP2AR: AACCGGTTGGTCGACTATTG
Experiment: Expression of different PEP1 transcripts in accession Dor ( Figure 5D, 5E, 5F )
PEP1_3UTRR1AaPP2AR PEP1(a+b)F: CTTGTCGTCTCCTCCTCTGG [PEP1(a+b)]PEP1(a+b)R: ACTACGGCGAGAGCAGTTTCPEP1aF: GCTTAGTATCTCAGGCGAC [PEP1a]PEP1R: TGCGACGTTTAGAGAAGGTGPEP1bF: GAACAACCGATATTGATGCTC [PEP1b]PEP1R: TGCGACGTTTAGAGAAGGTGAaPP2AF: AGTATCGCTTCTCGCTCCAGAaPP2AR: AACCGGTTGGTCGACTATTG
Experiment: 5′RACE
PEP1(a+b)R NESTR1: GATCATCTGCATGTCGTTTTCC