Figure 2 .

Most pfl mutants display physiological and/or molecular defects suggestive of peroxisomal defects. (A) Root lengths of 8-day-old pfl or Wt (Col-0) seedlings grown in yellow light in the presence or absence of sucrose or on sucrose-supplemented medium containing inhibitory concentrations of IBA or 2,4-DB are shown. Error bars show standard deviations of the means (n ≥ 12). (B) Hypocotyl lengths of 6-day-old pfl or Wt (Col-0) seedlings grown in the dark in the presence or absence of sucrose or on sucrose-supplemented medium containing inhibitory concentrations of IBA or 2,4-DB are shown. Error bars show standard deviations of the means (n ≥ 12). (C) Protein extracts from the 8-day-old seedlings grown in the light on 0.5% sucrose (in A) were processed for immunoblotting. The membrane was serially probed with antibodies to the indicated proteins. The positions of molecular mass markers (in kilodaltons) are indicated at the left. PMDH and thiolase (PED1) are synthesized as precursors (p) containing the PTS2 signal that is processed into the mature (m) protein in peroxisome. Residual PEX7 (PEX7) from a previous probing remains visible in the PMDH panel. HSC70 is a loading control. Experiments in A through C were repeated twice with similar results.