Figure 4 .

Histogram showing percentage mortality in the hybrids of transgenic Nistari and nontransgenic CSR2 lines. TAFib6, transgenic line that expresses nontarget dsRNA; IE, transgenic line with single viral gene target; MG(+), transgenic lines with segments of four viral target genes transcribed in sense orientation; MG(−), transgenic lines with segments of four viral genes transcribed in antisense orientation; MG(+/−), transgenic line with four essential baculoviral target genes expressing as a stem-loop structure to induce RNAi; and MG(+) × MG (−) transgenic lines obtained by crossing MG(+) and MG(−) lines. Third-instar larvae were infected per os at a dose of 6000 OBs/larva. Mortality was scored as number of moths succumbed to viral infection by counting the live moths eclosed from the infected larvae. Mortality was reduced significantly in lines having the multiviral genes. Bar indicates SD, N = 48. (**) P < 0.01; (***) P < 0.001; ns, not significant.