Table 4 . Comparative infectivity of OBs obtained from transgenic CSR2 and nontransgenic CSR2 larvae.
| 95% fiducial limit |
|||||||
|---|---|---|---|---|---|---|---|
| Type of OBs used | Test strain | LD50 | Upper | Lower | 1/slope | Regression equation | Standard error |
| OBs from transgenics | Nontransgenics | 4786 | 3801 | 6025 | 1.804 | Y = 0.5542X + 2.962 | 0.1013 |
| OBs from nontransgenics | Nontransgenics | 1698 | 1258 | 2398 | 1.146 | Y = 0.8728X + 2.176 | 0.1320 |
| OBs from transgenics | Transgenics | 21877 | 18197 | 25703 | 1.948 | Y = 0.5134X + 2.773 | 0.0747 |
| OBs from nontransgenic | Transgenics | 2570 | 2238 | 2951 | 1.822 | Y = 0.5488X + 3.127 | 0.0573 |
Day 1 fourth instar transgenic and nontransgenic CSR2 lines were fed per os with doses of OBs ranging from 1000 to 40,000 per larva. Thirty larvae per dose per line were used. The mortality was recorded from day 1 postinfection until pupation. The probit values were plotted against log doses for calculation of LD50 using GraphPad Prism 6 software. LD50 values of the transgenic and nontransgenic lines infected either with transgenic- or nontransgenic-derived OBs differed significantly (P < 0.05).