Figure 2 .

The iodine-staining phenotype of S. japonicus var. versatilis. (A) Picture of iodine-staining phenotype of colonies originating from random meiotic spores of the Sj4 strain. (B) Picture of iodine-staining phenotype of segregants of the Sj2 × Sj3 cross. The eight segregants from individual octads were arranged in a horizontal row on YEA growth medium by micromanipulation. The colonies of segregants dissected from seven asci are shown. After growth for 3 days, they were replicated onto plates containing PMA medium. After 3 days of growth and sporulation there, iodine-staining phenotype of colonies and culture patches was photographed. At the top, patches of heterothallic Sj2, Sj3, and homothallic Sj4 stocks are shown for comparing their staining phenotype.