Figure 3 .

The structure of S. japonicus mating-type loci. (A) Diagrams of the mat1 and donor loci: Sequence accession nos. JQ735907 and JQ735908. The proposed Imprint site (Imp) located at the end of the H1 element is indicated by a solid triangle. The probes used for Southern blot experiments (mat1M and H3) are shown in boldface lines. The physical distance and orientation of mat1 with respect to the donor loci are unknown. The EcoRI restriction enzyme sites are indicated with the letter E. The S. pombe genes situated around mat1 are indicated with open arrows. The S. japonicus retrotransposon homologs, with nucleotides identity >70%, that flank the donor loci, are shown with shaded arrows. (B) Sizes of the predicted Southern blot bands are listed. The bands resulting from the DSB due to mat1 imprint in a fraction of DNA molecules are marked with *. (C) Southern blot analysis of the mating-type loci. The genomic DNA was digested with EcoRI (left) and hybridized with the mat1M probe. The mat cassette fragment sizes are indicated. Right shows hybridization with the H3 probe. The size markers are indicated on the left side of both Southern blot panels.