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. 2012 Dec 20;8(12):e1003133. doi: 10.1371/journal.pgen.1003133

Figure 7. Overexpression of mct-1 extends lifespan.

Figure 7

A–D. Expression pattern of GFP-fused MCT-1 protein in mct-1p::mct-1::GFP transgenic animals. Bright field image (A) and green fluorescence image (B) of an L4 mct-1p::mct-1::GFP transgenic larva. Magnified bright field image (C) and green fluorescence image (D) of dotted box regions in A and B respectively. E–H. The expression of GFP-fused MCT-1 protein in the mct-1p::mct-1::GFP transgenic animal did not overlap with the signal from amphid sensory neurons labeled with the DiI dye. Bright field image (E), green fluorescence image (F), red fluorescence image (G) and overlay of green and red fluorescence images (H) of the head of an L3 mct-1p::mct-1::GFP transgenic animal. Arrowheads indicate cell bodies of amphid sensory neurons that are stained with DiI dye. The arrow indicates body wall muscle that expresses a muscle-specific myo-3p::RFP transgene, used as a marker for the generation of the transgenic animals. I–J. Expression pattern of GFP-fused MCT-1 protein in daf-10(m79) mutants, including representative green fluorescence and bright field images (I) and quantification of fluorescence intensity (J). Error bars represent s.e.m. (* p<0.05, Student's t-test). K. Overexpression of mct-1::GFP lengthens lifespan. Three independent lines of mct-1 transgenic animals lived slightly but significantly longer than control animals. Statistical analysis of the lifespan data presented in this figure and additional repeats is included in Table S1B.