Table 2. Genes down-regulated in daf-10(m79) mutants versus wild-type animals.
Gene | Brief description | p value | Fold change | qRT-PCR data | DAF-16 target? | DAF-12 target? | Pathogen-responsive? | Tissue expression? |
T21E8.2/pgp-7 | P-glycoprotein, ABC transporter | 7.97E-05 | 0.08 | Down in daf-10(m79) | G | Tail, intestine [67], [68] | ||
C05A9.1/pgp-5 | P-glycoprotein, ABC transporter | 2.44E-04 | 0.10 | Down in daf-10(m79) | B | G | Intestine [68] | |
K01A2.3 | Unknown | 0.004 | 0.14 | Intestine, muscle [69], [70] | ||||
C07G3.2/irg-1 | Riboflavin synthesis protein, DUF1768 | 0.003 | 0.15 | Not induced by PA14 in daf-10(m79) | F, G | Intestinal (enriched, I)Intestinal, induced by PA14 [71] | ||
B0024.1/col-149 | Collagen | 0.001 | 0.17 | |||||
Y49G5A.1 | Serine protease inhibitor | 0.002 | 0.18 | Down in daf-10(m79), osm-5(p813)Regulated by daf-12 | B* | D | G* | |
W06G6.12 | Unknown | 0.004 | 0.20 | |||||
T21E8.1/pgp-6 | P-glycoprotein, ABC transporter | 0.002 | 0.21 | G | Neuronal (expressed, H)Neurons, intestine [68] | |||
C32H11.12/dod-24 | Unknown, DUF141, Age | 0.002 | 0.21 | Down in daf-10(m79), osm-5(p813)Regulated by daf-12 | A, B | D | F, G | |
T24C4.4 | Unknown, DUF1164 | 0.003 | 0.23 | Down in daf-10(m79)Induction by PA14 is reduced in daf-10(m79) | B, C | F, G | Intestinal (expressed, I) | |
C32H11.4 | Unknown, DUF141 | 0.003 | 0.25 | Down in daf-10(m79), osm-5(p813)Induction by PA14 is reduced in daf-10(m79)Regulated by daf-12 | A, B, C | D | F, G | |
C10H11.6/ugt-26 | UDP-glucuronoyl transferase | 0.005 | 0.25 | B | G | |||
C03H5.1/clec-10 | C-type lectin | 0.004 | 0.26 | B | F*, G* | Intestinal (expressed, I) | ||
F55G11.2 | Unknown, DUF141 | 0.004 | 0.26 | Down in daf-10(m79), osm-5(p813)Regulated by daf-12 | B, C | D | F, G | |
F38A5.9/nspb-5 | Unknown, worm specific, DUF1459 | 0.018 | 0.27 | |||||
K10D11.1/dod-17 | Unknown, DUF141, Age | 0.007 | 0.27 | Down in daf-10(m79), osm-5(p813)Induction by PA14 is reduced in daf-10(m79)Regulated by daf-12 | A, B, C | D | F, G | Intestinal (expressed, I) |
T20D4.3 | Peptide N glycanase, worm specific, DUF750 | 0.009 | 0.28 | G* | ||||
C17H12.6 | Unknown, DUF141 | 0.007 | 0.29 | Down in daf-10(m79), osm-5(p813)Regulated by daf-12 | B | D | F, G | Intestinal (enriched, I) |
Y22D7AR.10 | Histone acetyltransferase | 0.006 | 0.29 | |||||
T20D4.5 | Peptide N-glycanase, DUF750 | 0.008 | 0.30 | |||||
F42A9.6 | Unknown, possible function in germline | 0.006 | 0.30 | Commonly expressed in germline, muscle and intestine (I)Neuronal (expressed, H) | ||||
Y53F4B.11 | Unknown | 0.011 | 0.31 | |||||
T02E1.7 | SURF4 family, putative cargo protein | 0.015 | 0.31 | |||||
Y45F10C.2 | Unknown, DUF1505 | 0.007 | 0.31 | Uterus [72] | ||||
F35E12.5 | Unknown, DUF141 | 0.008 | 0.31 | A, B, C | D | G | ||
Y69A2AR.25 | Unknown | 0.011 | 0.32 | Up in osm-5(p813) | D | |||
C29F9.14/C29F9.9 | Unknown, 7TM receptor | 0.009 | 0.32 | C* | ||||
ZK6.11 | Transmembrane glycoprotein, DUF274, regulates fat accumulation, aging | 0.011 | 0.32 | Down in daf-10(m79) | A, B, C | G | Intestinal (enriched, I) | |
F19B2.5 | Helicase-like transcription factor | 0.013 | 0.33 | F, G | Intestinal (expressed, I) | |||
C29F9.3 | Unknown | 0.010 | 0.33 | Intestinal (enriched, I) | ||||
F55G11.3 | Pseudogene | 0.014 | 0.34 | Down in daf-10(m79), osm-5(p813)Regulated by daf-12 | B | D | G | |
F31F4.15/fbxa-72 | F-box protein | 0.017 | 0.34 | Down in daf-10(m79), osm-5(p813)Regulated by daf-12 | B | D | G* | Intestinal (enriched, I) |
C08F11.12 | Unknown, DUF1505 | 0.015 | 0.35 | |||||
K11H12.4 | Unknown, DUF274, predicted GPI-anchor | 0.017 | 0.35 | Not repeated | B, C | G | ||
C17H12.8 | Unknown, DUF141 | 0.016 | 0.37 | A | G | Intestinal (enriched, I) | ||
C14C6.5 | ShK toxin domain | 0.015 | 0.38 | F | Intestinal (expressed, I) | |||
D1025.6/nspc-16 | Unknown, worm specific | 0.015 | 0.38 | |||||
C33C12.4 | Unknown, worm specific, Emb | 0.020 | 0.38 | Intestinal (expressed, I) | ||||
Y58A7A.3 | RNAse III family, Zn-finger protein | 0.018 | 0.39 | B | G | |||
T19D12.4 | Unknown, Esp | 0.017 | 0.40 | Down in daf-10(m79) | A. B | F, G | Intestinal (enriched, I) | |
Y69A2AR.13 | Unknown | 0.019 | 0.40 | |||||
C29F3.7 | Unknown, DUF141, Gro | 0.020 | 0.44 | B | F, G | Intestinal (enriched, I) | ||
T24B8.5 | ShK toxin domain | 0.019 | 0.56 | Down in daf-10(m79), osm-5(p813) | A, B | F | ||
R06C1.4 | RNA binding protein | 0.019 | 0.56 | B | Intestinal (expressed, I) | |||
C55B7.4/acdh-1 | Acyl CoA dehydrogenase, Esp, Age | 0.018 | 0.56 | F*, G* | Intestinal (expressed, I) | |||
C36C5.5 | Worm-specific Cys-rich secreted protein, DUF19 | 0.019 | 0.57 | |||||
F36D1.4 | Unknown | 0.019 | 0.57 | D* | ||||
C06G3.12 | Transposon | 0.019 | 0.57 | |||||
Y69E1A.8 | Unknown | 0.017 | 0.57 | |||||
F25H5.8 | Member of UPF0057, stress responsive protein | 0.018 | 0.57 | |||||
F38E1.3 | Protein kinase | 0.020 | 0.57 | |||||
ZK1251.1/htas-1 | Histone H2A variant | 0.017 | 0.57 | D* | Germline [73] | |||
T22A3.2/hsp-12.1 | Member of Hsp20 protein family | 0.019 | 0.57 | Muscle [69] | ||||
K08E7.4 | Unknown | 0.019 | 0.57 | |||||
K10C2.3 | Aspartyl protease | 0.020 | 0.57 | Down in daf-10(m79) | D | F, G | ||
C54F6.13/nhx-3 | Sodium/proton exchanger | 0.019 | 0.69 | D | Hypodermis, reproductive system [74] |
A = down-regulated in a daf-16-dependent fashion in daf-2 mutants, Murphy et al., 2003 [31].
B = down-regulated in a daf-16-dependent fashion in daf-2 mutants, McElwee et al., 2004 [35].
B* = up-regulated in a daf-16-dependent fashion in daf-2 mutants, McElwee et al., 2004 [35].
C = down-regulated in a daf-16-dependent fashion in daf-2 mutants, Lee et al., 2009 [34].
C* = up-regulated in a daf-16-dependent fashion in daf-2 mutants Lee et al., 2009 [34].
D = down-regulated by DAF-12, Fisher and Lithgow, 2006 [30].
D* = up-regulated by DAF-12, Fisher and Lithgow, 2006 [30].
F = up-regulated during PA14 exposure, Shapira et al., 2006 [32].
F* = down-regulated during PA14 exposure, Shapira et al., 2006 [32].
G = up-regulated during PA14 exposure, Troemel et al., 2006 [33].
G* = down-regulated during PA14 exposure, Troemel et al., 2006 [33].
H = Von Stetina et al., 2007 [23].
(“Expressed”: genes whose expression is detected in the neurons, “Enriched”: genes that are solely or more highly expressed in neurons).
I = Pauli et al., 2006 [22].
(“Expressed”: genes whose expression is detected in the intestine, “Enriched”: genes that are solely or more highly expressed in intestine).