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. 2012 Oct 12;12:185. doi: 10.1186/1472-6882-12-185

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Copyright ©2012 Duangmano et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cucurbitacin B changes nucleophosmin/B23 localization in human breast cancer cells. MCF-7 and MDA-MB-231 cells were stained with anti-nucleophosmin/B23 primary antibody. Alexa 568-conjugated anti-mouse IgG rabbit antibody was used as secondary antibody. DAPI was used for nuclear localization. Stained cells were analyzed by using confocal microscope. After exposure to 2.5 μM cucurbitacin B for 20 min. Translocation of nucleophosmin/B23 from nucleolus (red) to nucleoplasm (green) was observed.