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. 2012 Dec 20;8(12):e1003111. doi: 10.1371/journal.pgen.1003111

Figure 4. Distribution patterns and levels of TBP, of total RNA Pol II, and of Ser5P Pol II.

Figure 4

A) Relative TBP levels at the promoters of the WRKY70 and LTP7 genes in empty vector control (Con) plants, in AtWRD5-RNAi (wR1, wR2) lines (top panel) and the AtASH2-RNAi (a2R7, a2R16) lines (bottom panel). ACT 7 is used as an internal control; B–C) total Pol II and Ser5P distribution profiles along the genes in control (Con) and RNAi knockdown genotypes as specified above. Amplified regions are numbered as indicated in Figure 2A. ChIP-PCR values with IgG in all genetic backgrounds were <0.001 of the input levels (not shown). Each experiment was repeated at least three times. Each bar is standard errors of the mean (±SEMs, n = 3).