Figure 5. GATA-2 binding at the −566 GATA site of the ^Aγ-globin gene in wild-type β-YAC.

A) GATA-2 protein occupancy at the −566 ^Aγ-globin GATA site by ChIP analysis. B) GATA-2 protein occupancy at the murine Gata-2 gene −2.8 Kb binding site by ChIP analysis. Binding of GATA-2 at the murine Gata-2 locus was used as a positive control for GATA-2 occupancy. ChIP was performed as described in the legend to Figure 4; labeling is also as illustrated in Figure 4.