Table 4. Power calculation as a function of number of sequence reads and sample size for the comparison of ranked from the subgingiva and supragingiva populations, using as a reference the taxa frequencies obtained from the 24 samples, and 1% and 5% significant levels.
Alpha = 1% | Reads | |||||||
Subjects | 500 | 1000 | 2500 | 5000 | 10000 | 20000 | 50000 | 1000000 |
10 | 8.57% | 9.56% | 10.06% | 10.98% | 10.51% | 10.50% | 10.62% | 10.17% |
15 | 15.88% | 17.42% | 18.91% | 19.55% | 19.85% | 19.29% | 19.32% | 20.10% |
25 | 36.36% | 38.81% | 41.65% | 41.65% | 42.91% | 42.93% | 42.66% | 43.54% |
50 | 81.81% | 85.60% | 87.38% | 88.16% | 87.50% | 87.98% | 88.30% | 88.59% |