Table 1. Drugs sensitivity profile in L. donovani promastigote lines.
| Lines | Drugs EC50 (µM)-Resistance Index | |||
| AmB | MLF | PMM | SbIII | |
| WT | 0.07±0.01 | 5.84±0.43 | 12.09±1.76 | 87.33±5.72 |
| A | 0.14±0.04* (2.00±0.57) | 5.28±0.58 (0.90±0.16) | 19.67±1.30**** (1.63±0.34) | 74.38±4.98* (0.85±0.12) |
| M | 0.07±0.03 (1.00±0.56) | 10.56±1.06****,a (1.81±0.31) | 6.93±0.80 (0.57±0.15) | 92.23±7.09 (1.06±0.16) |
| P | 0.07±0.02 (1.00±0.42) | 4.98±0.38* (0.85±0.12) | 133.29±12.36****,b,c (11.02±1.63) | 138.15±15.54**** (1.58±0.29) |
| S | 0.07±0.02 (1.00±0.42) | 4.25±0.44*** (0.73±0.13) | 109.34±2.94**** (9.04±0.85) | 266.07±10.12****,d,e (3.04±0.32) |
| AM | 0.14±0.04* (2.00±0.86) | 18.03±1.07**** (3.09±0.40) | 19.35±3.67* (1.60±0.53) | 108.97±4.67**** (1.25±0.14) |
| AP | 0.14±0.04* (2.00±0.86) | 10.86±1.67**** (1.86±0.42) | 25.67±2.26**** (2.12±0.40) | 96.69±5.60 (1.11±0.14) |
| AS | 0.14±0.04* (2.00±0.86) | 5.79±0.40 (0.99±0.13) | 22.82±2.92**** (1.89±0.51) | 178.88±5.91**** (2.05±0.49) |
| MP | 0.07±0.03 (1.00±0.56) | 25.90±1.74**** (4.43±0.61) | 172.27±3.52**** (14.25±1.35) | 74.59±3.85** (0.85±0.10) |
| SP | 0.07±0.02 (1.00±0.42) | 4.61±1.02 (0.79±0.23) | 221.88±30.15**** (18.35±2.15) | 190.57±20.96**** (2.18±0.39) |
Promastigote forms of L. donovani lines: wild-type (WT) and resistant lines to AmB (A), MLF (M), PMM (P), SbIII (S), AmB+MLF (AM), AmB+PMM (AP), AmB+SbIII (AS), MLF+PMM (MP), and SbIII+PMM (SP), were grown as described in Materials and Methods section for 72 h at 28°C in the presence of increasing concentrations of drugs. Cell viability was determined using an MTT-based assay. Resistance indexes, between parentheses, were calculated by dividing the EC50 for each resistant line by that for WT line. Data are means of EC50 or resistance indexes values ± SD from three independent experiments. Significant differences versus the WT were determined using Student's t-test (*: p<0.05; **: p<0.01; ***: p<0.005; ****: p<0.001) and for comparison of singly versus doubly-resistant lines (a: p<0.001 M vs. AM or MP; b: p<0.05 P vs. MP or SP; c: p<0.001 P vs. AP; d: p<0.05 S vs. SP; e: p<0.005 S vs. AS).