Table 2. Drugs sensitivity profile in L. donovani intracellular amastigote lines.
| Lines | Drugs EC50 (µM)-Resistance Index | |||
| AmB | MLF | PMM | SbIII | |
| WT | 0.027±0.002 | 2.18±0.43 | 3.93±0.60 | 3.66±0.14 |
| A | 0.057±0.003****,a (2.110±0.270) | 2.03±0.33 (0.93±0.13) | 4.43±0.45 (1.13±0.28) | 2.38±0.19*** (0.65±0.07) |
| M | 0.028±0.002 (1.037±0.145) | 5.12±0.51*** (2.35±0.69) | 5.26±0.35 (1.34±0.29) | 5.11±0.46* (1.40±0.18) |
| P | 0.026±0.003 (0.963±0.180) | 1.98±0.23 (0.91±0.28) | 41.45±3.78****,b,c (10.55±1.57) | 4.66±0.35* (1.27±0.14) |
| S | 0.024±0.002 (0.889±0.140) | 2.08±0.17 (0.95±0.26) | 4.36±0.53 (1.11±0.30) | 44.47±4.23****,d,e (12.15±0.92) |
| AM | 0.064±0.002**** (2.370±0.251) | 4.62±0.39** (2.12±0.87) | 3.45±0.49 (0.88±0.26) | 3.36±0.23 (0.92±0.09) |
| AP | 0.058±0.003**** (2.148±0.273) | 3.98±0.41* (1.83±0.24) | 7.10±0.62**** (1.81±0.43) | 3.98±0.29 (1.09±0.09) |
| AS | 0.042±0.003** (1.556±0.342) | 3.46±0.54 (1.59±0.16) | 3.93±0.51 (1.00±0.28) | 6.61±0.57*** (1.81±0.22) |
| MP | 0.036±0.004 (1.333±0.256) | 7.09±1.07*** (3.25±1.31) | 41.58±3.44**** (10.58±1.49) | 3.48±0.27 (0.95±0.14) |
| SP | 0.026±0.004 (0.963±0.226) | 2.46±0.47 (1.13±0.23) | 64.48±5.34**** (16.41±1.84) | 14.05±2.04*** (3.84±0.69) |
Intracellular amastigote L. donovani lines were obtained after infection of mouse peritoneal macrophages with the different promastigote resistant lines as described in Material and Methods section. After 72 h, drug activity was determined from the percentage of infected cells and the number of amastigotes by cells in drug-treated cultures versus non-treated cultures. Resistance indexes, between parentheses, were determined as previously described in Table 1. Data are means of EC50 or resistance indexes values ± SD from three independent experiments. Significant differences versus the WT were determined using Student's t-test (*: p<0.05; **: p<0.01; ***: p<0.005; ****: p<0.001) and for comparison of singly versus doubly-resistant lines (a: p<0.05 A vs. AS; b: p<0.05 P vs. SP; c: p<0.001 P vs. AP; d: p<0.005 S vs. SP; e: p<0.001 S vs. AS).