Table 2. DNA sequencing and subsequent taxa assignments of the most prevalent Tsp509I terminal fragments (TFs) derived from 15 samples with different T-RFLP patterns.
| In silico TF in bp | In vitro TF in bp | Taxa assignation | Number of samples with TF (%) | Number of DNA Sequences | |
| 671 | 665 | Streptococcus spp. 1* | 105 (68.6) | 84 | |
| 541 | 535 | Moraxella spp. 1 | 48 (31.4) | 7 | |
| 524 | 518 | Corynebacterium spp. | 45 (29.4) | 32 | |
| 207 | 204 | Streptococcus spp. 2* | 32 (20.9) | 14 | |
| 469 | 464 | Haemophilus influenzae | 21 (13.7) | 3 | |
| 358 | 352 | Neisseria spp. | 13 (8.5) | 8 | |
| 153 & 156 | 148 | Moraxella spp. 2 & Sphingobacterium spp. | 9 (5.9) | 3 & 14** | |
| 558 | 550 | Staphylococcus spp. | 7 (4.6) | 8 | |
| 501 | 496 | Prevotella spp. | 5 (3.3) | 5 | |
| 195 | 190 | Streptococcus pyogenes | 5 (3.3) | 4 | |
| 561 | 552 | Gemella spp. | 4 (2.6) | 1 | |
| 591 | 583 | Granulicatella spp. | 4 (2.6) | 3 | |
| 551 | 544 | Stenotrophomonas maltophilia | 3 (2.0 | 8 | |
| 190 | 185 | Chrysebacterium spp. 1 | 3 (2.0) | 6 | |
| 702 | 697 | Chrysebacterium spp. 2 | 2 (1.3) | 6 | |
| 204 | 199 | Microbacterium spp. 1 | 2 (1.3) | 14 | |
| 374 | 371 | Fusobacterium spp. | 2 (1.3) | 1 | |
| 169 | 167 | Turicella spp. | 1 (0.7) | 6 | |
| 549 | 540 | Klebsiella spp. | 1 (0.7) | 1 | |
| 645 | 640 | Propionibacterium spp. | 1 (0.7) | 3 | |
| 209 | 206 | Porphyromonas spp. | 1 (0.7) | 1 | |
| 529 | 524 | Microbacterium spp. 2 | 1 (0.7) | 1 | |
| TOTAL | 153 (100) | 233 | |||
*
Streptococcus pneumoniae and pseudopneumoniae are included in Streptococcus spp. 1 while other members of the Mitis group are included in Streptococcus spp. 2. Using the second restriction enzyme (Hpy166II) Streptococcus spp. 1 can be further divided into S. pneumoniae and S. pseudopneumoniae (see text).
**
Two different bacterial assignments were retrieved for TF with 148 base pairs (bp).