Figure 1. Study of the energy-dependent endocytosis of fluorescent nanodiamonds in NIH/3T3 EWS-Fli1 cells.

The fNDs (red channel) loaded with FITC-labeled siRNA (green channel) were observed by confocal microscopy (Leica TSC SPE) after 4 h of incubation. We used 50 nM siRNA vectorized with ND-PAH or ND-PEI at mass ratios of 1∶25 and 1∶75, respectively. Cells were incubated at 37°C (control) (A, B), 4°C (C, D), or 37°C after treatment for 30 min treatment with NaN₃ (E, F). Nuclei are labeled with DAPI (blue channel), scale bars: 20 µm.