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The cells were treated with 50 nM antisense siRNA vectorized with ND-PEI at a mass ratio of 1∶75, with or without drugs specifically inhibiting particular internalization pathways: chlorpromazine, blocking the clathrin pathway and amiloride, inhibiting the macropinocytosis pathway. EWS-Fli1 gene expression is normalized with respect to that of the 18S rRNA gene.
