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. 2012 Dec 20;7(12):e52150. doi: 10.1371/journal.pone.0052150

Figure 1. A schematic view of the tofI, tofM, and tofR loci and the DNA constructs used for deletion mutation and genetic complementation.

Figure 1

The grey areas indicate the flanking regions cloned in pKKSacB for individual or combined deletions of tofI, tofM, and tofR. The genomic regions to be deleted with the DNA constructs in pKKSacB are indicated with vertical hatched lines, while those cloned in a broad host vector, pBBR1MCS-2 or pBBR1MCS-5, for complementation tests are indicated with horizontal solid lines. Small arrows indicate the primers (Table 2) used for the amplification of each flanking region. Abbreviation for restriction sites are as follows: Bg, BglII; E, EcoRI; EV, EcoRV; K, KpnI; N, NotI; P, PstI; Pv, PvuII; S, SacI; Sa, SalI; SII, SacII; X, XhoI. Restriction sites used for generating pBBtofIMR, pBBtofIM, and pBBtofRM are denoted with parentheses.