Figure 3. Toxoflavin production by Burkholderia glumae strains, 336gr-1 (wild type), LSUPB145 (ΔtofI), LSUPB169 (ΔtofR) and LSUPB139 (ΔtofI-tofR) in the presence or absence of 1.

µM N- hexanoyl homoserine lactone (C6-HSL) and N- octanoyl homoserine lactone (C8-HSL) in LB broth (A) and on LB agar (B). LB broth and LB agar were inoculated with equal amounts of bacterial cells (∼10⁶ CFU) per ml of media. Bacteria were spread uniformly on LB agar plates with a spreader. Photos were taken and toxoflavin were quantified after 24 h incubation at 37°C. LB agar plates were photographed after removal of bacterial culture from the medium. Error bars indicate the standard deviation from three replications.