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. 2012 Dec 20;7(12):e51672. doi: 10.1371/journal.pone.0051672

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© 2012 Aillet et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) In vitro SUMOylation assay using IκBα WT or mutated on lysines 21 and 22 as substrates. (B) HEK293 cells were transfected with the indicated plasmids, pre-treated or not with MG132 and stimulated or not with TNFα. His₆-ubiquitylated or SUMOylated proteins were purified using denaturing conditions and Ni²⁺ chromatography.