Figure 5. Integration of SUMO molecules into IκBα Ubiquitin chains.

Seventy-two hours after transfection with control or Ubc9 siRNA (100 nM), HeLa cells were pre-treated with MG132, stimulated 20 min with TNFα and lysed in a buffer containing TUBE-hHR23A. TUBE-captured material was eluted and submitted to IκBα immunoprecipitation. Western blot detection with (A) anti-IκBα, (B) anti-ubiquitin, (C) anti-SUMO2/3 and (D) anti-sam68 and anti-Ubc9 antibodies.