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. 2012 Dec 20;7(12):e51495. doi: 10.1371/journal.pone.0051495

Figure 2. Time course of SG formation.

Figure 2

A, Drosophila S2R+ cells were exposed to oxidative stress and SGs were visualized by FISH. B, ER-stress was induced in U2OS cells and SGs were visualized by immunofluorescence of eIF3η. Confocal images (63×, 1024×1024 pixels) were analyzed manually or with BUHO with a scaling down to 512×512. Ten micrographs from triplicate wells (A) and 5 micrographs from duplicates (B) were analyzed at each time point. Error bars indicate standard deviation. Size bar, 10 µm.