Table 1. Summary of manual and script analysis of images used for BUHO adjustment.

Treatment	Cell number		SG number		Cells with SGs		SGs per cell
	Manual	BUHO	Manual	BUHO	Manual	BUHO	Manual	BUHO
basal	18	18	0	0	0	0	0	0
basal	16	16	0	0	0	0	0	0
basal	21	21	0	0	0	0	0	0
stress 1	20	20	56	52	12	11	4.7	4.3
stress 2	20	20	40	40	11	11	3.6	3.6
stress 3	24	24	40	42	12	12	3.3	3.3
stress 4	18	18	12	12	5	5	2.4	2.4
stress 5	16	16	47	47	8	8	5.4	5.4
stress 6	27	27	87	89	17	17	5.1	5.2
Summary 1–6	125	125	282	280	65	64	4.3	4.4

Nine images from wells treated or not with arsenite (training set) were manually analyzed. Cells were considered positive when they contain two or more SGs [33]. Average number of SGs in the SG-positive cells is indicated. These values were used to tune the MATLAB script and values computed after BUHO optimization with this training set are indicated.