Table 3. Summary of manual and BUHO analysis for the identification of SG regulators.
dsRNA | Cells with SGs (%) | SGs per cell | ||
Manual | BUHO | Manual | BUHO | |
none | 37 | 37 | 3.6 | 3.6 |
none | 47 | 47 | 4.5 | 4.5 |
none | 40 | 40 | 4.4 | 4.4 |
none | 42 | 43 | 4.0 | 3.9 |
none | 40 | 41 | 5.5 | 5.4 |
none | 42 | 42 | 3.9 | 3.8 |
LacZ | 42 | 42 | 4.2 | 4.2 |
LacZ | 42 | 42 | 4.3 | 4.3 |
LacZ | 41 | 43 | 4.7 | 4.8 |
GCN2 | 29 | 28 | 4.2 | 4.2 |
GCN2 | 37 | 38 | 4.4 | 4.4 |
GCN2 | 26 | 23 | 3.5 | 3.5 |
PP1α | 59 | 58 | 5.0 | 4.9 |
PP1α | 54 | 52 | 4.2 | 4.2 |
PP1α | 53 | 57 | 4.3 | 4.3 |
FAK 56D | 53 | 52 | 4.8 | 4.8 |
FAK 56D | 57 | 54 | 5.1 | 5.1 |
FAK 56D | 61 | 60 | 4.6 | 4.6 |
S2R+ cells were treated with the indicated dsRNA and exposed to arsenite. SG formation was analyzed in 7 images (test set) with an average of 300 cells for each treatment. Manual values and BUHO generated results are indicated.