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. 2012 Dec 20;7(12):e51495. doi: 10.1371/journal.pone.0051495

Table 3. Summary of manual and BUHO analysis for the identification of SG regulators.

dsRNA Cells with SGs (%) SGs per cell
Manual BUHO Manual BUHO
none 37 37 3.6 3.6
none 47 47 4.5 4.5
none 40 40 4.4 4.4
none 42 43 4.0 3.9
none 40 41 5.5 5.4
none 42 42 3.9 3.8
LacZ 42 42 4.2 4.2
LacZ 42 42 4.3 4.3
LacZ 41 43 4.7 4.8
GCN2 29 28 4.2 4.2
GCN2 37 38 4.4 4.4
GCN2 26 23 3.5 3.5
PP1α 59 58 5.0 4.9
PP1α 54 52 4.2 4.2
PP1α 53 57 4.3 4.3
FAK 56D 53 52 4.8 4.8
FAK 56D 57 54 5.1 5.1
FAK 56D 61 60 4.6 4.6

S2R+ cells were treated with the indicated dsRNA and exposed to arsenite. SG formation was analyzed in 7 images (test set) with an average of 300 cells for each treatment. Manual values and BUHO generated results are indicated.