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. 2012 Dec 20;7(12):e51707. doi: 10.1371/journal.pone.0051707

Figure 5. Platelet inhibition prevents MMP activity and reactive oxygen species (ROS) formation in mouse aortas.

Figure 5

A. In situ zymography for gelatinase activity. Aortas from Ang II-treated and Ang II+clopidogrel-treated mice were analyzed. B. Densitometric analysis of MMP activity by DQ gelatin (a fluorogenic substrate used to detect protease activity) in aortas from Ang II-treated (n = 3) and Ang II+clopidogrel-treated (n = 3) mice. *P<0.05 compared with Ang II-treated mice only. C. In situ dihydroethidium (DHE) staining of aortas from Ang II-treated and Ang II+clopidogrel-treated mice. Green fluorescence in the media, observed in both groups, is due to elastin fiber autofluorescence. All sections are shown with the lumen at the top. D. Densitometric analysis of DHE fluorescence in aortas from Ang II-treated (n = 3) and Ang II+clopidogrel-treated (n = 3) mice. *P<0.05 compared with Ang II-treated mice only. Bar = 50 µm.