Skip to main content
. 2012 Dec 20;7(12):e51716. doi: 10.1371/journal.pone.0051716

Figure 2. Assessment of MELOE-1 CD4 T cell responses in PBMC from healthy donors.

Figure 2

PBMC from healthy donors were stimulated with 10 µM of MELOE-1. After 14 days, the presence of CD4 T cells specific for the different regions of MELOE-1 was assessed by re-stimulating cells with MELOE-12–21, MELOE-111–30, MELOE-118–37 and MELOE-126–46 peptides, followed by CD4/TNF-α double staining and flow cytometry analysis. Between brackets is indicated the mean % of TNF-α producing CD4 T cells, in positive microcultures. NA: not available.