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. 2012 Dec 20;7(12):e51716. doi: 10.1371/journal.pone.0051716

Figure 4. MELOE-1 specific responses in melanoma patients.

Figure 4

(A) Dot plot illustrating peptide-specific Th1 responses in MELOE-1 stimulated microcultures from Pt≠3. Fourteen days after PBMC stimulation with MELOE-1 whole polypeptide (2–46), microcultures were re-stimulated with each indicated peptide during 5 h. Specificity was then assessed by a triple labelling IFN-γ/IL-4/CD4. Percentages indicate the fraction of IFN-γ producing T cells, among CD4 T cells. (B) Frequency of microcultures containing IFN-γ (black bars) or IL-4 (white bars) producing CD4 T cells specific for MELOE-1 overlapping peptides. A microculture was considered as positive when the fraction of cytokine producing CD4 T cells was 2-fold higher upon peptide stimulation than in unstimulated cultures. (C) Percentages of cytokine producing CD4 T cells in positive microcultures from all patients. IFN-γ (left panel) and IL4 (right panel) production was assessed by a triple labelling IFN-γ -IL4-CD4, after peptide re-stimulation.