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. 2012 Dec 20;7(12):e51716. doi: 10.1371/journal.pone.0051716

Figure 5. Assessment of MELOE-1 CD4 T cell responses in PBMC from melanoma patients.

Figure 5

PBMC from melanoma patients were stimulated with 10 µM of MELOE-1. After 14 days, the presence of CD4 T cells specific for the different regions of MELOE-1 was assessed by re-stimulating cells with MELOE-12–21, MELOE-111–30 and MELOE-122–46 peptides, followed by CD4/IFN-γ double staining for the detection of Th1 responses, and by CD4/IL4 double staining for Th2 responses. Between brackets is indicated the mean % of cytokine producing CD4 T cells, in positive microcultures.