Table 1. TCR characterization and cytokine profile of MELOE-1 specific CD4 T cell clones.
| MELOE-12–21 specific CD4 T cell clone (9C12- DQß1*0202) | ||
| CDR3 beta chain | Cytokine profile a | |
| V beta chain | Vß2.1 | TNF high |
| CDR3 beta | CSA SPDTHWGTDTQ YFG | IFN high |
| J beta chain | Jß 2.3 | IL2 high |
| GM-CSF high | ||
| IL4 high | ||
| IL5 low | ||
| IL13 high | ||
| IL10 neg | ||
| MELOE-111–30 specific CD4 T cell clone (1A5–DRß1*1101) | ||
| V beta chain | Vß5.1 | TNF high |
| CDR3 beta | CAS SSAGGNSGNTI YFG | IFN high |
| J beta chain | Jß 1.3 | IL2 high |
| GM-CSF high | ||
| IL4 low | ||
| IL5 neg | ||
| IL13 neg | ||
| IL10 neg | ||
| MELOE-111–30 specific CD4 T cell clone (5F9-DRß1*0101) | ||
| V beta chain | Vß8.2 | TNF high |
| CDR3 beta | CAS SRTGGNYGY TFG | IFN high |
| J beta chain | Jß1.2 | IL2 low |
| GM-CSF high | ||
| IL4 high | ||
| IL5 neg | ||
| IL13 high | ||
| IL10 neg | ||
| MELOE-126–46 specific CD4 T cell clone (4E2- DQß1*0201) | ||
| V beta chain | Vß2.1 | TNF high |
| CDR3 beta | CSA SGRRKFYEQ YFG | IFN high |
| J beta chain | Jß 2.7 | IL2 high |
| GM-CSF high | ||
| IL4 high | ||
| IL5 low | ||
| IL13 high | ||
| IL10 neg | ||
a
CD4 T cell clones were stimulated for 5 hours in the presence of brefeldin A (10 µg/mL) either with the cognate peptide (10 µM) in an autopresentation assay. After 5 hours of stimulation, cells were stained with APC-conjugated anti-CD4 mAb, fixed with 4% paraformaldehyde, labeled with PE-conjugated anti-cytokine mAb and analyzed by flow cytometry. A “high” cytokine production was defined with a threshold of 20% of producing CD4 T cells, in response to the peptide.