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. 2012 Dec 20;7(12):e50638. doi: 10.1371/journal.pone.0050638

Figure 7. Anti-tumor activity of Sal in vivo.

Figure 7

A. HE staining showed the structure of the liver cancer tissue: nuclei of different sizes, hepatic cord structure was destroyed, Magnification 200×. B. Immunohistochemistry indicates that PCNA expression is down-regulated after Sal treatment. Magnification 200×. C.HE staining showed apoptotic and necrotic areas(nucleus disintegration, organizational integration) increased after Sal treatment. Magnification 100×. TUNEL apoptotic assay indicates increased apoptosis in Sal-treated tumor tissue. Immunohistochemistry shows that Bcl-2 expression was down-regulated and Bax expression was up-regulated after Sal treatment. Magnification 200×. D. Sal inhibits Wnt/β-catenin signaling pathways. Expression of p-GSK-3β, GSK-3β, and β-catenin was evaluated by Western blot. Immunohistochemistry showed that β-catenin expression was down-regulated after Sal treatment. Magnification 200×.