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. 2012 Dec 20;7(12):e51610. doi: 10.1371/journal.pone.0051610

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© 2012 Ramírez-Latorre

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Perforated whole-cell current of a SN DA neuron at P0. The whole cell patch was obtained and the current was recorded for five minutes to assay for decay. After 5 min, 20 mM TEA was applied in a fast perfusion system. No appreciable diminution of the current was observed. The slice was then washed. After the wash, 1 M IbTx was applied. No effect was observed on the outward current. The voltage protocol is indicated below the trace.

Inline graphic — Perforated whole-cell current of a SN DA neuron at P0. The whole cell patch was obtained and the current was recorded for five minutes to assay for decay. After 5 min, 20 mM TEA was applied in a fast perfusion system. No appreciable diminution of the current was observed. The slice was then washed. After the wash, 1 M IbTx was applied. No effect was observed on the outward current. The voltage protocol is indicated below the trace.