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. 2012 Dec 20;7(12):e51610. doi: 10.1371/journal.pone.0051610

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© 2012 Ramírez-Latorre

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Perforated whole-cell patch on a P15 SN DA neuron. Currents are in points, fits are continuous lines. Protocol identical to the one used in figure 6. Control trace (marked CONTROL) is shown before the application of 20 mM TEA. (B) The application of TEA reduces the current to the TEA insensitive current (I ). The trace shown is obtained from panel A middle after a capacitance subtraction. This TEA-insensitive current has the characteristic ms activation seen in figures 5 at P0. This current was fit according to the equation . This fit is superimposed on the whole-cell current as a continuous line. Two time constants were necessary to fit the data, a voltage independent fast component of 4–7 ms, and a slow one of 85 ms (V from −20 to 100 mV, step 20 mV, the slow component was fit by = 85, 85, 85, 86, 88, 84, and 85 ms respectively). (C) Two components can be detected in the I current of the SN DA neuron. A step depolarization to +20 mV (C left), or +40 mV (C right) produces two types of currents that are sensitive to TEA. The I component is characterized by a voltage dependent activation and a voltage independent inactivation time constant ( ms). The I component is characterized by a voltage dependent activation time constant. The lines are the fits to the I current shown. In the left trace, (activation) ms, and in the right hand side, (activation) ms. A second voltage dependent component with slower time constants was used to fit these data ( ms, lhs panel and ms, rhs panel).

Inline graphic — (A) Perforated whole-cell patch on a P15 SN DA neuron. Currents are in points, fits are continuous lines. Protocol identical to the one used in figure 6. Control trace (marked CONTROL) is shown before the application of 20 mM TEA. (B) The application of TEA reduces the current to the TEA insensitive current (I ). The trace shown is obtained from panel A middle after a capacitance subtraction. This TEA-insensitive current has the characteristic ms activation seen in figures 5 at P0. This current was fit according to the equation . This fit is superimposed on the whole-cell current as a continuous line. Two time constants were necessary to fit the data, a voltage independent fast component of 4–7 ms, and a slow one of 85 ms (V from −20 to 100 mV, step 20 mV, the slow component was fit by = 85, 85, 85, 86, 88, 84, and 85 ms respectively). (C) Two components can be detected in the I current of the SN DA neuron. A step depolarization to +20 mV (C left), or +40 mV (C right) produces two types of currents that are sensitive to TEA. The I component is characterized by a voltage dependent activation and a voltage independent inactivation time constant ( ms). The I component is characterized by a voltage dependent activation time constant. The lines are the fits to the I current shown. In the left trace, (activation) ms, and in the right hand side, (activation) ms. A second voltage dependent component with slower time constants was used to fit these data ( ms, lhs panel and ms, rhs panel).