Figure 12. RNS60 protects mice from EAE via Tregs.

A) MBP-primed T cells isolated from MBP-immunized donor mice were re-primed with MBP in the presence of 10% v/v RNS60 for 2 d followed by purification of CD4+CD25+ Tregs. In a parallel experiment, EAE was induced in female SJL/J mice by adoptive transfer of MBP-primed T cells and from 4 dpt, mice were treated (i.p.) once with RNS60-induced MBP-primed Tregs (1×10⁶ Tregs/mouse). Purified CD4+CD25− MBP-primed non-Tregs were also used for comparison. Five mice were included in each group. Mice (n = 5) were examined for clinical symptoms daily until 36 dpt. B) EAE was induced in female SJL/J mice by adoptive transfer of MBP-primed T cells and from 1 dpt, mice were treated with RNS60 (dpt 1–8, alternate day; dpt 9–16, daily; dpt 17 onwards, alternate day) followed by one i.p. injection of anti-CD25 antibody (50 µg/mouse) on 2 dpt. One group of mice also received same amount of control IgG. Mice (n = 5) were examined for clinical symptoms daily until 33 dpt.