Figure 5. TGF-β inhibition reverses gastric cancer cell- exosomes induced Smad2 and p38 activation in hucMSCs.

A. HucMSCs were treated with SGC7901 derived exosomes (800 µg/mL) in the presence or absence of SD208 (2 µM) for 1 hour. The levels of phosphorylated Smad2 and p38 were examined by Western blotting. B. Gastric cancer cell (SGC7901) derived exosomes were pre-incubated with anti-TGF-β antibody (20 µg/mL) for 2 h, and then added to hucMSCs. Six hours later, the cells were collected and the levels of phosphorylated Smad2 and p38 proteins were examined by Western blotting.