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. 2012 Dec 20;7(12):e51767. doi: 10.1371/journal.pone.0051767

Figure 1. Characterization of Panx1−/− mice.

Figure 1

a, RT-PCR and western blot analysis of 4 adult Panx1+/+ control and Panx1−/− animals. Note the lack of the 287 bp PCR amplicon representing the deleted exon4 in the Panx1 mRNA and the loss of the Panx1 protein in Panx1−/− mice. b, Immunohistochemistry demonstrating loss of Panx1 protein expression in CA1 region of the hippocampus and cerebellum. Abbreviations: M, muscle, H, heart, B, brain, R, retina, OR, stratum oriens, PY, stratum pyramidale, RAD, stratum radiatum, GCL, granular cell layer, PCL, Purkinje cell layer, ML, molecular layer. Scale bars: 100 µm.