Table 1. Summary of SSU rDNA barcode analysis of nematodes isolated from flowerbed and agricultural field soils.
rOTUa | No. of isolates | Nematodes belonging to each rOTUb | Feeding typesc | Accession no. |
K01rOTU01 | 18 | SnTUT_k01_01, 05, 06, 08, 11, 12, 16, 18, 19, 24, 26, 29, 44, 52, 54, 65, 69, 75 | plant feeding (1d) | AB728372–AB728389 |
K01rOTU02a | 18 | SnTUT_k01_03, 07, 14, 21, 32, 41, 42, 45, 51, 56, 63, 64, 66, 67, 68, 74, 76, 80 | animal predation (5a) | AB728390–AB728407 |
K01rOTU02b | 6 | SnTUT_k01_22, 43, 55, 58, 59, 62 | animal predation (5a) | AB728408–AB728413 |
K01rOTU03 | 6 | SnTUT_k01_23, 33, 46, 49, 60, 61 | animal predation (5) | AB728414–AB728419 |
K01rOTU04 | 4 | SnTUT_k01_28, 40, 53, 73 | plant feeding (1e) | AB728420–AB728423 |
K01rOTU05 | 3 | SnTUT_k01_02, 20, 48 | bacterial feeding (3) | AB728424–AB728426 |
K01rOTU06 | 3 | SnTUT_k01_25, 36, 71 | animal predation (5) | AB728427–AB728429 |
K01rOTU07 | 2 | SnTUT_k01_70, 77 | plant feeding (1d) | AB728430, AB728431 |
K01rOTU08 | 1 | SnTUT_k01_34 | plant feeding (1d), animal predation (5), omnivorous (8) | AB728432 |
K01rOTU09 | 1 | SnTUT_k01_35 | bacterial feeding (3) | AB728433 |
K01rOTU10 | 1 | SnTUT_k01_37 | plant feeding (1), hyphal feeding (2)? | AB728434 |
K01rOTU11 | 1 | SnTUT_k01_39 | animal predation (5), omnivorous (8) | AB728435 |
K01rOTU12 | 1 | SnTUT_k01_47 | - | AB728436 |
K01rOTU13 | 1 | SnTUT_k01_50 | - | AB728437 |
K01rOTU14 | 1 | SnTUT_k01_57 | - | AB728438 |
K01rOTU15 | 1 | SnTUT_k01_72 | - | AB728439 |
H01rOTU01 | 8 | SnTUT_h01_02, 15, 21, 29, 38, 41, 43, 46 | bacterial feeding (3) | AB728324–AB728331 |
H01rOTU02 | 6 | SnTUT_h01_05, 19, 24, 26, 27, 40 | bacterial feedingd | AB728332–AB728337 |
H01rOTU03 | 6 | SnTUT_h01_22, 25, 30, 48, 54, 55 | bacterial feeding (3) | AB728338–AB728343 |
H01rOTU04 | 5 | SnTUT_h01_08, 23, 47, 50, 52 | bacterial feeding (3) | AB728344–AB728348 |
H01rOTU05 | 5 | SnTUT_h01_03, 14, 16, 33, 37 | bacterial feeding (3) | AB728349–AB728353 |
H01rOTU06 | 5 | SnTUT_h01_10, 13, 42, 51, 53 | bacterial feeding (3) | AB728354–AB728358 |
H01rOTU07 | 3 | SnTUT_h01_20, 44, 45 | bacterial feeding (3) | AB728359–AB728361 |
H01rOTU08 | 2 | SnTUT_h01_09, 35 | bacterial feeding (3) | AB728362, AB728363 |
H01rOTU09 | 2 | SnTUT_h01_04, 18 | animal predation (5a) | AB728364, AB728365 |
H01rOTU10 | 2 | SnTUT_h01_31, 32 | - | AB728366, AB728367 |
H01rOTU11 | 2 | SnTUT_h01_36, 39 | hyphal feeding (2) or plant feeding (1e) | AB728368, AB728369 |
H01rOTU12 | 1 | SnTUT_h01_01 | - | AB728370 |
H01rOTU13 | 1 | SnTUT_h01_17 | animal predation (5) | AB728371 |
Codes “K01”, “H01” and “rOTU” represent “flowerbed sample with experimental code” , “agricultural field sample with experimental code” and “SSU rDNA-derived OTU”, respectively. Two-digit serial numbers were assigned in order of the number of nematodes in the rOTU.
Nematodes isolated from flowerbed and agricultural field soils were designated as SnTUT_k01_a two-digit serial number and SnTUT_h01_a two-digit serial number, respectively.
Feeding types were derived from those of nematode species with the highest sequence homologies by Blast search according to the references in Yeates et al. (1993) [41]. Numbers and letters in parentheses indicate feeding types in the reference.
Feeding type for H01rOTU02 was derived from the other reference [44].