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. 2012 Dec 20;7(12):e52450. doi: 10.1371/journal.pone.0052450

Figure 2. Endothelial-specific effects of anti-Dll4 treatment.

Figure 2

(A) Immunofluorescence for CD105 and c-kit (Zeiss AxioImager.Z1). Arrowhead, CD105+c-kit− blood vessel; arrow, CD105+c-kit+ blood vessel. Bar = 20 µm. (B) c-kit+(CD105+) vessel percentage, per high power field (200x, Zeiss AxioImager.Z1), reveal an increase of c-kit+ BM vessel percentage in anti-Dll4 treated mice. (C) Angiocrine gene modulation was accessed by relative quantification of mRNA from total BM, revealing a decrease in FGF1 and CSF2 and an increase of IGFbp2, IGFbp3, Angpt2, Dll4, DHH and VEGF-A expression in anti-Dll4 treated mice. (D) HUVEC phosphorylation of Akt and ERK1/2, analysed by Western blotting. (E) Quantification of phosphorylation of Akt and ERK1/2 relative band intensity reveals a significant increase of Akt phosphorylation. Data are means ± s.e.m. *, p<0.05; n = 3.