Figure 6. Geldanamycin induces degradation of the CaM KMT.

HeLa cells transfected with Myc-CaM KMT (A) and with FLAG-CaM KMT (B) cells were treated with increasing concentrations of GA for 24 h, followed by immunoblotting with monoclonal antibodies anti-Myc (A) and anti-FLAG (B) to examine protein levels of CaM KMT. Western blot with anti-GAPDH antibody was used as proteins’ loading control. The results shown in (A) are representative of four independent experiments. The results shown in (B) are representative of two independent experiments.