Figure 2. RWC and expression of TaAQP7 in wheat seedlings with different treatments.

Ten-day-old wheat seedlings were subjected to 12 h dehydration treatment and the wheat leaves were sampled to measure RWC (A) and the expression of TaAQP7 (B). Ten-day-old wheat seedlings were treated with 20% of PEG6000 (C), 100 µM ABA (D), and 10 mM H₂O₂ (E) and leaves were sampled within 24 h to extract RNA for qRT-PCR analysis. The relative fold difference in mRNA level was calculated using the 2^–ΔΔCt formula with TaActin as internal control. The mRNA fold difference was relative to that of distilled water treated samples used as calibrator. Vertical bars indicate ±SE of four replicates on one sample. Asterisks indicate significant difference between the RWC or relative fold difference in mRNA level (*p<0.05; **p<0.01). When no bar is shown, the deviation is smaller than the symbol. Three biological experiments were performed, which produced similar results.