Figure 8. Analysis of H₂O₂ accumulation, SOD and CAT activities in transgenic lines under osmotic stress.

The WT and transgenic lines were cultured in MS medium under a 16 h light/8 h dark cycle at 25°C for one week, and then the seedlings were transplanted to MS or MS with 300 mM mannitol for one week. The whole seedlings were used to detect H₂O₂ accumulation, SOD and CAT activities. (A) In situ detection of H₂O₂ by DAB staining of WT and transgenic seedlings. (B) H₂O₂ content. (C) SOD activity. (D) CAT activity. Vertical bars indicate ±SD calculated from four replicates. Asterisks indicate significant difference between the WT and the three transgenic lines (*p<0.05; **p<0.01). Three biological experiments were performed, which produced similar results.