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Ssl1 migrates at 33 kDa. It was expressed in E coli-CodonPlus (DE3)-RP and purified to homogeneity by heat precipitation and immobilized metal affinity chromatography (IMAC). M: molecular size marker, lane 1: cell extract before induction, lane 2: cell extract after expression, lane 3: soluble fraction from cell disruption, lane 4: soluble fraction from heat precipitation, lane 5: IMAC eluate.
