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. 2012 Dec 20;7(12):e52356. doi: 10.1371/journal.pone.0052356

Figure 4. Silencing of Cdk2 and suppression of MCF-7 cell proliferation.

Figure 4

The effectiveness of the plasmid DNA encoding Cdk2 shRNA was tested on MCF-7 cells growing on tissue culture plates, both in terms of suppressing Cdk2 expression, as measured by Q-PCR on culture day 4 (A) and cellular proliferation, as measured by the MTS assay on culture day 1 (B). The effect on proliferation and cell death was also examined via microscopy in the three conditions (C). The plasmid DNA encoding for EGFP shRNA was used as a control in both experiments. Control indicates results from untransfected cells. (A) *p<.03, **p<.05. (B) *p<.001, **p<.001.