Figure 7. Nucleolar translocation of RPS9 1–70 domain by co-expressed NPM1.

A.) Nucleolar localization of EGFP-RPS9^1–70 was induced by co-expressed Myc-NPM1 (wt), whereas a Myc-NPM1 mutant lacking its nuclear localization signal (Myc-NPM1ΔNLS) failed to do so. Cells were stained for expression of wt and mutant Myc-NPM1. B.) Myc-NPM1 (wt) or Myc-NPM1 mutant did not induce any alteration in the localization of EGFP-RPS9^140–194. Bar 10 µM. U2OS cells were transfected with plasmids as indicated in the figure and fixed 24 hours after transfection. Cells were stained for Myc expression. Corresponding merged and DAPI images are also shown. Bar 10 µM.