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. 2012 Dec 20;7(12):e52483. doi: 10.1371/journal.pone.0052483

Figure 4. Inhibition of JNK suppressed the ABT-737-induced Bim expression.

Figure 4

HeLa cells were treated with ABT737 for 24 h, in the absence or presence of 10 µM SP600125. Then cells were lysed and the indicated proteins were analyzed by Western Blotting (A). Real-time RT-PCR showed Bim mRNA expression in HeLa cells was blocked by SP600125 (B). HeLa cells were transfected with negative control or JNK siRNA for 48 h, then treated with or without ABT737 for 24 h. The cells were lysed and the indicated proteins were analyzed by Western Blotting (C). Real-time showed that silencing of JNK by siRNA blocked ABT737-induced Bim mRNA expression in HeLa cells (D). HeLa cells were transfected with negative control or c-jun siRNA for 48 h, then treated with/without ABT737 for 24 h. The cells were lysed and the indicated proteins were analyzed by Western Blotting (E). Real-time showed that silencing of c-jun by siRNA blocked ABT737-induced Bim mRNA expression in HeLa cells (F). Error bars represent standard deviations (SD) calculated from three parallel experiments (**, p<0.01; #, p>0.05).