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. 2012 Dec 20;7(12):e52568. doi: 10.1371/journal.pone.0052568

Figure 2. Influence of miR-203 on Hakai levels in several cell lines.

Figure 2

A, the effect of modulating miR-203 levels in 293 cells was studied as described in 1D and 1E; Western blot analysis (left panel) and quantification by densitometry (right panel). B, the effect of modulating miR-203 levels in A549 cells was studied as described 1D and 1E. Western blot analysis (left panel) and quantification by densitometry (right panel). C, endogenous Hakai expression levels in human colorectal cell lines (SW480 and SW620), as assessed by western blotting (upper panel) and by RT-qPCR (lower panel) analysis. D, endogenous miR-203 levels in human colorectal cell lines (SW480 and SW620) by RT-qPCR (lower panel). Values (A–D) are the means ± SEM from three independent experiments. Significant differences in the indicated transfected miRNA with respect to transfected scrambled control (Ctrl) miRNA for A–B, and the between SW480 and SW620 cell lines for C–D are indicated (*p<0.05, **p<0.01, ***p<0.001, n = 3). The western blotting data are representative of three independent experiments.