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. 2012 Dec 20;7(12):e52644. doi: 10.1371/journal.pone.0052644

Figure 2. RNA editing in trans-splicing nad1e branched molecules.

Figure 2

(A) Scheme of the branched splicing intermediate formed during trans-splicing. The position of the primers used to isolate the splicing intermediate are indicated by red and black arrows. Dotted lines indicate the nucleophilic attack of the 2′OH from the bulged A of domain D6 to obtain the 2′-5′-branched intermediate (B) Gel electrophoresis of the PCR products obtained by combining primers P2a and P3a (nad1d/e). The sequence of primers used to isolate nad1e and nad5c splicing intermediates is indicated in the File S1. The electropherograms of selected cloned PCR product is shown. Red arrowheads indicate the edited U generated by the conversion of the genome encoded C residue. The asterisk signals the A residue at the branching point; this residue is missing in the sequence electropherogram. M, PhiX174 DNA/HaeIII (Promega) molecular weight markers.