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. 2012 Dec 20;7(12):e52644. doi: 10.1371/journal.pone.0052644

Figure 3. RNA editing in trans-splicing nad5c branched molecules.

Figure 3

(A) Scheme of the nad5b and mat-r-nad1e-nad5c transcripts. The dimension of boxes do not represents the actual size of the exons. The primers used for branched splicing intermediate analysis are indicated by arrowheads. (B) Agarose gel electrophoresis analysis of RT-PCR products from the nad5c branched structure. cDNA was synthesized with primer P1b, followed by PCR amplification using primers P2b and P4 (lane 1) or primers P2b and P5 (lane 2). The primer P4 is specific of the maturase Dx domain and P5 is specific of exon nad1e. M, molecular weight markers. (C) Selected parts of the electropherogram of a 1.5 kbp cloned PCR product are shown. The sequence starting from the maturase domain Dx and the beginning of the 3′ half of the nad1-I4 intron (upper panel), the end of the 3′ half of the nad1e intron and the nad1e exon (middle panel) and the nad5c branched splicing intermediate (lower panel) are shown. Red arrowheads show the position of edited residues.